Virus Pathogen Database and Analysis Resource (ViPR) – Herpesviridae – Protein Details for UL47 –

Establishment of real-time quantitative reverse transcription polymerase chain reaction assay for transcriptional analysis of duck enteritis virus UL55 gene. et al. et al. et al. et al. et al. Establishment of real-time quantitative reverse transcription polymerase chain reaction assay for transcriptional analysis of duck enteritis virus UL55 gene.

Establishment of real-time quantitative reverse transcription polymerase chain reaction assay for transcriptional analysis of duck enteritis virus UL55 gene. Sanderson, M. Establishment of real-time quantitative reverse transcription polymerase chain reaction assay for transcriptional analysis of duck enteritis virus UL55 gene. The Chinese virulent (CHv) strain of duck enteritis virus (DEV) has a genome of approximately 162,175 nucleotides with a GC content of 44.89%. et al. Establishment of real-time quantitative reverse transcription polymerase chain reaction assay for transcriptional analysis of duck enteritis virus UL55 gene. Li Y.,Huang B.,Ma X.,Wu J.,Li F.,Ai W.,Song M.,Yang H.

et al. et al. et al. The loss of viability was also dependent on the MOI at which the cultures were infected (Figure A). Establishment of real-time quantitative reverse transcription polymerase chain reaction assay for transcriptional analysis of duck enteritis virus UL55 gene. et al. Establishment of real-time quantitative reverse transcription polymerase chain reaction assay for transcriptional analysis of duck enteritis virus UL55 gene.


Li Y.,Huang B.,Ma X.,Wu J.,Li F.,Ai W.,Song M.,Yang H. et al. From each dilution, 1 μL was used as a template and subjected to the conventional PCR and FQ-PCR protocol. The eye inspection method was simple and rapid, but offered difficulty in detecting quantitative amplification. Li Y.,Huang B.,Ma X.,Wu J.,Li F.,Ai W.,Song M.,Yang H. et al. et al.

Li Y.,Huang B.,Ma X.,Wu J.,Li F.,Ai W.,Song M.,Yang H. characteration of the newly identified duck enteritis virus ul55 gene product has not been reported yet. et al. Li Y.,Huang B.,Ma X.,Wu J.,Li F.,Ai W.,Song M.,Yang H. The directed movement of HSV-1 and PRV UL37-null capsids is substantially impeded, which supports the idea that efficient capsid transport to the organelle of secondary envelopment requires assembly of UL37 onto capsids (6, 26). et al. et al.

et al. Additionally, the requirement for the mardivirus-specific genes LORF4A and LORF5 are reported for the first time. After herpesvirus infections were documented, antiviral treatment with acyclovir was initiated as well. Many viruses inducing apoptosis by modulating expression of Bcl-2 family proteins have been reported [27]. and Haynes, J.S. Copyright © 2012, American Society for Microbiology. Although nonessential for virus infectivity of cultured cells, gC is a highly antigenic glycoprotein, of which the importance in eliciting immune responses has been well documented for many herpesviruses[35-39].

Our previous study also showed that chitosan significantly improved CD4+ and CD8+ T cell responses to at least 6 weeks post-injection in Balb/c mice[36]. Secondly, our TIEM analysis showed that an association of DEV pUL51-specific labeling with cytoplasmic virions and also with some membranous structure observed near the intracellular virion. The recovery of infectious MeHV-1 from three UL21 transposed clones, Tn5Δ14, MuAΔ37 and MuAΔ41, in combination with the presence of viral DNA after sequential passages, confirms that MeHV-1 UL21 is non-essential for replication in cell culture. The levels of the mRNA transcripts of UL31 were determined by reverse transcriptase polymerase chain reaction (RT-PCR) on total RNA, extracted from uninfected or DEV-infected cells at different times p.i. Trypsinization of 9–11-day-old duck embryonated eggs was performed according to standard practice. Moreover, attenuated strains of Salmonella are attractive candidates for mucosal vaccine vectors, because they elicit both mucosal and systemic immune responses against carried antigens [39, 40]. Attenuated DEV vaccine strains, including C-KCE and clone03, are ideal avian vaccine vector candidates because these viruses can induce long-lasting protection against DEV in ducks, and they have a natural host range limited to avian species [39].

S. The theca folliculi from each duck were also used for virus isolation or detection by PCR. Liver: Necrosis, acute, random, multifocal and individual cell, marked, with intranuclear inclusion bodies, necrotizing vasculitis, hemorrhage and hepatocellular vacuolar change. We firstly reported the detection of DuCV in Fujian Province, China [19]. Four lines of evidence suggest that these atypical genes are truly part of the ChHV5 genome: (1) the pTARBAC insertion interrupted the UL52 ORF, leaving parts of the gene to either side of the insertion and suggesting that an intact molecule had been cloned. Contributor’s Comment: Pacheco’s disease (PD) is caused by a heterogeneous group of psittacid herpesviruses (PsHVs) closely related to Gallid herpesvirus-1.1-3 Since its initial description in Brazil in 1929, Pacheco’s disease has attained worldwide distribution with many reported outbreaks in the United States.1 Recent investigations in Europe and the United States indicate that there are at least 5 serologic serotypes and 10 genetic variants, and that infection by one serotype/variant may not be protective against infection by other variants.1-2 In spite of the heterogeneity, some variants occur more commonly than others. DEV, officially named anatid herpesvirus 1, belongs to the genus Mardivirus, family Herpesviridae [11].