Quiescent viral genomes in human fibroblasts after infection with herpes simplex virus type 1 Vmw65

(B) Immunoblots of cells infected with HSV-1(F). Taken together, these results demonstrate that ribosomal proteins are still synthesized up to the late time of infection and efficiently assembled into mature ribosomes, while there is a severe shutoff of the synthesis of other cellular proteins. The results indicate that HSV-1 infection of human endothelial cells produces complex effects on host-cell metabolism. PAA-treated and HSV-infected mice with nondetectable levels of antibodies did not develop, with a single exception, a latent ganglionic infection unpon reinfection. Cells infected with d95 at a multiplicity of infection of 10 PFU per cell retained a relatively normal morphology and expressed genes from the viral and cellular genomes for at least 3 days postinfection. In cells pretreated with IFN-alpha and subsequently infected with in1820 cytotoxicity was overcome, enabling a tissue culture system to be developed in which all cells stably retained at least one quiescent viral genome. The levels of GABA in GAD67 vector-infected cells were almost twofold higher than in GAD65 vector-infected cells.

Induction was mediated by a pathway other than the mechanism through which interferon-alpha mediates its effects on cellular gene expression. The induction of autophagy occurred when cells were infected with HCMV or HSV-1 that was gradient purified, but HCMV dense bodies and HSV-1 light particles, each of which lack nucleocapsids and genomes, were inactive. Cells infected with d95 at a multiplicity of infection of 10 PFU per cell retained a relatively normal morphology and expressed genes from the viral and cellular genomes for at least 3 days postinfection. Comparison of exonuclease and endonuclease activity in the presence of various divalent cations revealed that, at concentrations of Mn(2+) greater than 1 mM, only endonuclease activity occurs whereas endonuclease and exonuclease activity occur at all concentrations of Mg(2+). Changes in resting membrane properties, such as resting potential, input resistance and capacitance, remained small after the infection. Combined, these data show that, in some types of infection, CTL priming can extend well beyond the first 24-48 h after primary inoculation. The results indicate that HSV-1 infection of human endothelial cells produces complex effects on host-cell metabolism.


Reinoculation of ACG-treated mice at a site different from that of the primary inoculation did not lead to the establishment of a second latent infection with the homologous virus type when a latent infection was already present. Received April 20, 1977. In both viral infections, the neuron seemed to be the target cell at the early stages. In the presence of Mg2+, Ca2+ and Zn2+ are inhibitory. Cells infected with d95 at a multiplicity of infection of 10 PFU per cell retained a relatively normal morphology and expressed genes from the viral and cellular genomes for at least 3 days postinfection. Changes in resting membrane properties, such as resting potential, input resistance and capacitance, remained small after the infection. All HZ infections except 1 were localized to a single dermatome.

Inhibition of host cell DNA methylation may be an important step in the transformation of cells by herpesviruses, and various transformed cell lines tested showed reduced levels of DNA methylation. The rate of reaction at the optimal Mg2+ concentration is 3- to 5-fold greater than that at the optimal Mn2+ concentration. Relative to HSV-2/gD, percentages of HSV-specific CD8(+) T-cells were similar or only slightly reduced after infection with the mutant strain HSV-2/gD-Δ7-15, in all tissues up to 9 days after infection. The levels of GABA in GAD67 vector-infected cells were almost twofold higher than in GAD65 vector-infected cells. (C) Schematic representation of the experimental design similar to that shown in Fig. (B) Schematic representation of the experimental design. 1998 Dec; 71(6):2304-12.

Two hours after infection steady-state levels of the S-phase-specific cyclin, cyclin A, increased. The optimum concentration for Mn2+ is 0.1 to 0.2 mM and no exonuclease activity is observed when the concentration of Mn2+ is greater than 1 mM. The protein and its mRNA are easily recognized at 4 days by electrophoresis in high percentage acrylamide gels and by hybridization to cDNA, respectively. Consistent with this, TFH maintained high-level surface expression of CD69, indicative of impaired migratory capacity. The “moving wall” represents the time period between the last issue available in JSTOR and the most recently published issue of a journal. Specific lysis of HSV-infected cells was mediated by BW cells as early as 4 days after intratracheal infection of the rabbits with the virus whereas lysis by spleen cells and BALT cells was not detected until 7 or more days after infection. Circadian disruption, as occurs during shift work, increases the risk of chronic diseases.

Using a standard medium based on Dulbecco’s Modified Eagle Medium (D-MEM), no virus replication was observed in freshly isolated PEC. An immunohistological study of viral antigen (VA) in the brain was carried out in mice which had been infected with herpes simplex type 1 virus (HSV) in the skin of the face.