Molecular identification of human enteroviruses associated with aseptic meningitis in Yunnan province, Southwest China

Four hundred and fifty-six patients with signs and symptoms of potential central nervous system infection were evaluated from June 28, 1978, to September 30, 1978. Enteroviruses were detected in 12 (eight males, four females) out of 118 patients (10.16%). Connect to ProQuest Once connected, you can view documents in full as well as cite, email or print them. An outbreak of aseptic meningitis was recorded in Greece during the year 2001. This study analyzed the epidemiology, circulating pattern, and clinical presentations of CVB3 in Hong Kong over the last 10 years with reference to the circulation of EV in the locality. There were 286 and 169 cases, respectively. Attack rates for enterovirus-like illness were highest among varsity football team members (53%), and most of this illness occurred between 6 and 15 October.

In 2005, an outbreak of aseptic meningitis occurred in Seoul, Korea, which appeared to be caused by certain enterovirus strains. More than 90% of all aseptic meningitis cases are assumed to be viral, and the other 10% are bacterial. No, it’s not oral rehydration therapy or ondansetron – try a mnemonic! In samples, stored at -70°C, analyzed without repeated thawing, enteroviral RNA was demonstrable in 21/22 CSF specimens from which an enterovirus had been isolated. Cerebrospinal fluid samples (CSF) of 122 patients were found to contain enterovirus RNA using diagnostic RT-PCR that targeted a 231-bp gene fragment in the 5′ noncoding region. Focal encephalitis is increasingly recognized as a complication of enterovirus infection. Echovirus 16 was recovered from a throat swab of one hospitalized player and from the CSF of another.

VP4 is located on the internal side of the capsid. In addition, the journal features highly focused brief reports, review articles, editorials, commentaries, and supplements. Connect to ProQuest Once connected, you can view documents in full as well as cite, email or print them. Rotbart, University of Colorado School of Medicine, Pediatric Infectious Diseases, 4200 East 9th Avenue, Box C227, Denver, Colorado 80262. It was most similar to a strain of the same serotype, E30-14125-00, in the 5′ half of the genome but was almost equidistant to all strains analyzed in most of the 3′ half of the genome. For epidemiological surveillance, 342 enterovirus samples obtained from patients with aseptic meningitis in Belgium from 1999 to 2002 were first diagnosed as being enterovirus positive by amplification of the 5′ noncoding region (5′NCR) by reverse transcription (RT)-PCR. Enterovirus polymerase chain reaction (PCR) testing was positive on CSF from all three newborns.

In this study the procedure for Nested-PCR amplification of the VP1 gene was optimized. 36, 93; 1962, v. This is then cleaved co- and post- translationally by viral proteases to give the viral structural and non-structural proteins. The field covered is broad and includes the zoonoses, tropical infections, food hygiene, vaccine studies, statistics and the clinical, social and public-health aspects of infectious disease. ECHO 30 is also known to be the most frequent enterovirus causing outbreaks or sporadic cases of aseptic meningitis world-wide [4–7]. This is important since ~34% of various in-house RT-PCR assays developed for enterovirus detection have been reported to fail quality testing [17]. Abstract INTRODUCTION: Clinical and laboratory features of enteroviral meningitis may overlap with those of bacterial meningitis.

Echovirus 18 (Echo 18) is a member of the genus Enterovirus, family Picornaviridae. Cell culture was inoculated immediately and incubated for 14 days. STUDY DESIGN: In total, 213 CSF and faecal samples were examined for EV presence by culture, while enteroviral RNA detection was performed by nucleic acid sequence-based amplification assay (NASBA). For the case-crossover design, we established the hazard and non-hazard periods as week one and week four before admission, respectively. METHODS: Eighty-one patients (ages >or=15) with aseptic meningitis or encephalitis treated in the Infectious Diseases Hospital of Thessaloniki, Greece, during 2003-2006, were included in the study. An EV infection was diagnosed in 85/524 children (16.2 %) and the viruses detected were assigned to 16 serotypes. In this study, a total of 524 children were admitted to the children Kunming hospital (continental China) for aseptic meningitis manifestations in 2009 and 2010.

We have also shown that subgrouping of the isolates by RFLP assay based on the VP1 region is possible. Cerebrospinal fluid (CSF) specimens were obtained with criteria of aseptic CNS infections as documented by pleocytosis, negative Gram stain and negative bacterial culture. in 7.12% (n=8) by the microagglutination test; and arbovirus in non of the cases by inhibition of passive hemagglutination. Triplet B required one bolus of crystalloid and a brief course of supplemental oxygen therapy for episodes of bradycardia, which resolved promptly. During the past 11 years from 1981 through 1991, a total of 8,595 enterovirus isolations from aseptic meningitis cases were reported from 54 participating laboratories. This study was carried out to determine the incidence of Enteroviruses among aseptic meningitis with emphasis on Enterovirus 71 (EV71) in Khartoum, Sudan.