Molecular characterization of enteroviruses isolated from patients with aseptic meningitis in Korea, 2005

When these unexpected findings were associated with a presumptive clinical diagnosis of aseptic meningitis, watchful observation and repeat lumbar puncture precluded the necessity to administer antibiotics in every case. compares the laboratory findings of neonates and children with EVM. Nucleotide sequence analysis showed the existence of different genetic groups on the basis of the 5′-untranslated region (5′-UTR) of the genome, which circulated in the population during the same time period. Review of data from 1999 to 2008 showed increased activity of CVB3 in the years 2005 and 2008, and isolates in these 2 years displayed an amino acid change from threonine to alanine at codon 277 of the VP1 gene, which may be associated with central nervous system (CNS) disease. Twelve of these strains were grouped together with recent strains isolated in China and France. Since EV-71 replicates in the brain, neurological symptoms and ailments are common: headaches, mood swings, depression, anxiety, and bi-polar disease. Find many more like this one for viruses, bacteria, parasites, and fungi in First Aid for the USMLE Step 1 2013!

In the total clinical material of 169 patients, 67 (40%) were found positive by PCR, whereas an enterovirus was isolated from CSF in 54 (32%) cases. Other enteroviruses encountered were echovirus 16 (5.7%), echovirus 18 (1.6%), coxsackievirus B4 (0.8%) and echovirus 7 (0.8%). Membership on the school football team was associated with an increased attack rate of illness and a greater risk for aseptic meningitis developing. The P2 and P3 regions encode the nonstructural proteins associated with replication. Note: In calculating the moving wall, the current year is not counted. E30 is one of the two most widespread serotypes in the United States and is commonly associated with aseptic meningitis outbreaks (2). Several serotypes existed as multiple genotypes (subtypes) from 1999 to 2002, but genomic differences mainly resided at synonymous sites; these results strongly suggest that the subtypes exhibit similar antigenic properties.


Cerebrospinal fluid specimens were collected from 118 children younger than 13 years of age with clinical suspicion of viral meningitis [fever, vomiting, headache, signs of meningeal inflammation, CSF WBC count > 5 × 106/mL, and a negative CSF culture for bacterial infection (Tuberclosis)]. No long-term follow-up was performed in the current study. There are 36 serotypes of Human enterovirus B – Human coxsackievirus A9, Human coxsackievirus B1–B6, Human echovirus 1–7, 9, 11-21, 24-27, 29-33, Human enterovirus 69. The “moving wall” represents the time period between the last issue available in JSTOR and the most recently published issue of a journal. ECHO 13 was not isolated during 1996–1999, however it was the predominant serotype in Germany in 2000 (Tab. Few reports based on virus isolation procedures have found a much lower frequency of enterovirus meningitis in Jordan and Tunisia [22, 23]. A positive polymerase chain reaction (PCR) result was associated with relatively short hospitalization.

The patient had acute onset of fever, headache, nausea, and vomiting. Six specimens were positive by RT-PCR and negative by the culture method. All ECV4 isolates presented a 98.7% similarity in nucleotide sequence, with a Spanish ECV4 strain, isolated during a meningitis outbreak in 2006. Etiology was identified in 8 of 45 (18%) patients with encephalitis, EV (4/45, 9%) and HSV-1 (4/45, 9%) being the most common pathogens. This work indicates the need to perform large-scale surveillance to gain a better insight into the epidemiology of enteroviruses associated with aseptic meningitis in China. Following an unremarkable 1-month stay in the newborn intensive care unit, they were sent home in August with their parents, a 20-month-old brother who attended daycare, and the grandparents. This study has been conducted to identify the prevalent serotypes of enteroviruses in young children with aseptic meningitis during 2009 – 2010 in Iran.

During the epidemics, enterovirus-associated meningitis was most frequently reported among children of 4-7 years of age. The collected specimens were assayed to detect Enteroviruses and EV71 RNA using reverse transcriptase polymerase chain reaction (RT-PCR) technique. They were coxsackievirus (C) types B3 and B5, echovirus (E) types 4, 6, 7, 9, 18 and 30. Echovirus 9 (E9) and echovirus 30 (E30) have been associated frequently with outbreaks of aseptic meningitis. Identical genetic sequences were later obtained from cerebrospinal fluid of a patient with aseptic meningitis and a recent history of tick attachment. Sequence based typing was performed on the enterovirus positive samples to gain a better understanding of the molecular characteristics of the Coxsackie A9 (CVA-9) strain responsible for most cases in this outbreak. It has emerged as a major cause of a vast variety of diseases such as epidemics of hand, foot and mouth disease (HFMD), aseptic meningitis (AM), acute flaccid paralysis, and encephalitis.

However, there is limited information about HEV associated with aseptic meningitis in mainland China because it has not been classified as a notifiable disease. In the latter case, please turn on Javascript support in your web browser and reload this page.