Acute and fatal herpesvirus infection in a young Asian elephant (Elephas maximus). Gaskin, G. Gel electrophoretic separation of first-round PCR products by using Codehops primers LGH2595/2597 (550 bp). A second stage of the analysis was aimed at expanding the data available for two prototype strains each of EEHV1A and EEHV1B by PCR sequencing directly on amplified necropsy tissue DNA using numerous appropriate partially redundant primers designed based on conserved features of the available EEHV2 or EEHV1A reference sequences from the phage clones. Some animals will repeatedly lay down and stand up. The ORFs are listed in projected order from left to right across the genome and with provisional equivalent map coordinates given based on where they match to the recent complete draft 177,316-bp DNA sequence now available for EEHV1A(Kimba) as reported by Ling et al. Phylogenetic comparisons of the viruses from all nine Indian cases with selected prototype EEHV1A, EEHV1B, EEHV2, and EEHV6 cases at the U71-gM, vGPCR1, HEL, and gH-TK loci are presented in , and graphic alignment presentations of the nucleotide polymorphism patterns for the same four variable loci are presented in .
Despite the lower-than-expected overall GC content, the level does increase to an average of 62% (range, 57 to 66%) for the 17 largest protein coding regions, which are all over 2,500 bp in length and occupy 67.6 kb. Kiupel, R. Once this information is available, it is likely that dosing recommendations for famciclovir will require adjustment. Grobler,D.G., Raath,J.P., Braack,L.E.O., Keet,D.F., Gerdes,G.H., Barnard,B.J.H., Krick,N.P.J., Jardine,J., and Swanepoet,R. Very limited data exists on the pharmacokinetics of enrofloxacin in elephants2 and most of the dosage regimes for gastrointestinal absorption are based on horse dosages since they share a similar gastrointestinal tract. Order Herpesvirales, p 99–107 In King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ, editors. Often more than one male will gather around the area of a female that is ready to breed, and the most dominant male is the one who is allowed to carry out breeding.
(AP) — Justin Johnson scored 26 points on 12-for-17 shooting and grabbed 14 rebounds while Que Johnson made 8 of 12 shots and added 22 points as Western Kentucky handled UAB in a 76-64 win on Sunday. This strain supplies tRNAs for 7 rare codons (AGA, AGG, AUA, CUA, GGA, CCC, and CGG) in order to improve the expression of heterologous proteins. Four of the eight elephants in the Houston zoo’s herd – Thai, Methai, Tess and Shanti – are mature adults and unlikely to get sick from EEHV. (Carlsbad, CA). The elephant DNA can then be compared to DNA of confiscated ivory to help trace the ivory’s origin. Like Retraction Watch? Using a rapid and sensitive test based on real-time PCR, it has been determined that captive Asian elephants from several herds frequently shed EEHV1 in trunk secretions, and these secretions are a likely mode of viral transmission between animals.
Mac’s mother, Shanti, and the matriarch of the herd, Methai, hovered over his body. She soon realized that if she took a few courses, she could turn her research into a Ph.D. Dunker, C. If the St. Wild populations are restricted to increasingly limited areas of land and are under pressure with regard to habitat loss, poaching, human invasion and various diseases. The latter requires the elephant to ingest liquid through its trunk and then force out the water into a plastic bag. However, herpesviruses do not typically cause lethal infections in their natural hosts and factors contributing to EEHV-associated disease susceptibility remain enigmatic.
Schmitt lead the team which resulted in first elephant born from artificial insemination in 1999 and in his more than 30 years as an elephant veterinarian he has experienced over 50 elephant births some of which were born at the Ringling Bros. In the past four years since the collaboration was officially announced in 2009 under the leadership of Dr. Learn more about the viruses, the research and how to help in the fight against EEHVs here. Ellie is mother to Maliha, 6, and Rani, 16, and grandmother to Rani’s calves, Jade, 6, and Kenzi, who is almost 2. San Rafael, Calif. The capsid is transported to the nuclear pore where the viral DNA is released into the nucleus. Chai’s death is the second the zoo has experienced in recent months.
Since we have discussed these conservation threats in detail in previous blogs (feel free to scroll through to read about them!), I will focus on a few other causes of elephant death. Once removed from their families and natural habitat, their lives consist of little more than chains and intimidation. Guscetti, A. The 54-year-old Packy has shown a recurrence of tuberculosis, and treatment has stopped working and it�€�s been suspended. Scheduled to open in late spring the expansion will highlight the zoo’s global commitment and ongoing work to save elephants in the wild.