Kaposi’s sarcoma-associated herpesvirus infection of bone marrow dendritic cells from multiple myeloma patients. – PubMed

Slides were then washed twice with 0.1% Tween in PBS. HV is characterized by widened mantle zones composed of concentric rings of small lymphocytes in an “onion skin” pattern around small atrophic germinal centers with penetrating hyalinized vessels and dysplastic follicular dendritic cells (FDCs).1 In PC, the germinal centers are hyperplastic rather than atrophic, the interfollicular region contains sheets of plasma cells and vascular proliferations, the FDC network is normal, and there is preserved lymph node architecture.20 The mixed variant displays features of both HV and PC. The patient underwent a thoracocentesis for therapeutic relief as well as for diagnostic interpretation. However, the grave exceptions to the rule are unborn babies, as well as immunocompromised individuals. Herrada J, Cabanillas F, Rice L, et al. After informed consent and counseling, mothers were interviewed briefly and asked about social, demographic, and health-related factors. Briefly, the slides were deparaffinized, dehydrated, predigested with Pronase (Sigma, St Louis, MO), and hybridized overnight at a concentration of 0.25 ng/µL of probe.

To our knowledge, 12 cases of PT-PEL and 11 cases of PT-MCD have so far been observed. 49-year-old HIV-positive man presented with fever, malaise, and respiratory symptoms, subsequently diagnosed with HHV-8–associated CD. Both thoracentesis and paracentesis were performed. Immunocytochemistry recognized atypical CD20+ lymphoid cells [Figure 2]. That the infection may be transmitted through saliva or other types of casual contact has been suggested by seroepidemiological studies which have reported a high prevalence of HHV-8 infection among children in Sub-Saharan Africa and, to some extent, in Mediterranean countries [11,12]. The disease is confined to a single lymph node with UCD, while MCD typically presents with lymphoid hyperplasia at multiple sites, including the peripheral lymph nodes, bone marrow, and multiple organs. We believe that the measurement of viral DNA load in cell culture supernatants is an appropriate and valid tool to evaluate the antiviral drug susceptibility of EBV, HHV-6, and HHV-8 only when tested by fully controlled qPCR assays.


Nat. Monini for invaluable help in the development of serological testing; Ms. Seropositive and seronegative patients were followed for 16 months. The idea behind this experiment was that if a virus causes KS, the genomic DNA in the two samples should be precisely identical except for DNA belonging to the virus. Viral studies related HHV-6 seroprevelance in retinoblastoma patients would be useful to clarify if there is any etiological association between HHV-6 and retinoblastoma. Human herpes virus-8 or Kaposi’s sarcoma herpesvirus (HHV-8/KSHV), a γ-herpes virus closely related to Epstein–Barr virus (EBV), was first identified about a decade ago [1]. The patient was treated with multiple cycles of doxorubicin and paclitaxel leading to KS remission, while the lymphadenopathy and splenomegaly remained stable on follow-up CT examinations.

Human herpesvirus 8 (HHV-8) is recognized as a major causal agent of Kaposi sarcoma (KS), and it has been detected in all epidemiologic variants of KS. Skin involvement is frequently observed, though lungs, gastrointestinal tract and lymph nodes may also be affected. Furthermore, sequences of the entire ORF K1 of 4 strains showed that these HHV-8 strains of African origin were of the A5 or the B sub-type. ↵* Corresponding author. Collectively, these novel findings demonstrated that host-encoded miR-498 and miR-320d regulated HSV-1 induction of KSHV lytic replication by targeting RTA, which provided further insights into the molecular mechanisms controlling KSHV lytic replication. A 39-year-old male was referred to the First Affiliated Hospital of Wenzhou Medical College (Wenzhou, China) with a one-week history of abdominal pain and distension. The patient was treated with rituximab and THP-COP regimen, and achieved remission.

Because of the advanced age, no chemotherapy was given. Serum samples were tested for HHV-8 antibodies with use of an enzyme immunoassay against K8.1. Fixed, paraffin-embedded tissue sections from 21 cases of Kaposi sarcoma, nine cases of spindle cell hemangioma, five cases of cutaneous angiosarcoma, five cases of dermatofibrosarcoma protuberans, one case of vascular transformation of a lymph node, four cases of pilar leiomyoma, four cases of stasis dermatitis, four cases of pyogenic granuloma, and three cases of spindled melanoma were examined immunohistochemically using the rat monoclonal antibody to human herpes virus 8 latent nuclear antigen-1, open reading frame-73 (Advanced Biotechnologies Inc.). Both prevalence rates increased with age, and were lower in the villages near the Bensbach River. Lymph node biopsy confirmed HHV-8–associated CD and excluded KS and lymphoma. We report herein an unusual case of KSHV-associated HPS in an immunocompetent patient. NK cells recognize these targets through membrane receptors, which can trigger activating or inhibitory signals for killing.

The Viral Activation Transfusion Study was a prospective, randomized, double-blind comparison of transfusion with WBC-reduced versus non-WBC-reduced RBCs to HIV+ patients. However, limited information on epidemiologic patterns of HHV8 and HSV2 among MSM is available in mainland China. Warning: The NCBI web site requires JavaScript to function. Your donation makes a difference. PNAS is the world’s most-cited multidisciplinary scientific serial.