Histopathology and ultrastructural pathology of cyprinid herpesvirus II (CyHV-2) infection in gibel carp, Carassius auratus

The distribution in this summary table is based on all the information available. In young carp it can be fatal, and it has been linked to skull abnormalities. Whether hybrid common carp, other cyprinids, or non-cyprinid species can harbor KHV and later transmit the disease to naïve common carp varieties remains controversial. KHV begint zich te vermenigvuldigen bij een watertemperatuur vanaf 15°C. Microsatellites have been used to characterize different isolates, particularly in herpes cytomegaloviruses [13,14] and more recently in herpes simplex viruses [15] and in Ostreid herpes virus 1 [16]. Our results show that elimination of viral mRNAs proceeds in a specific hierarchy not correlated with the reappearance of the mRNA molecules following infection. The divergence existing between CyHV-3 IL-10 and carp IL-10 excludes the hypothesis that the peptides detected could be derived from carp IL-10 rather than from CyHV-3 ORF134.

As a result of our analysis, a substantial amount of information was garnered on transcript layout, and the AngHV1 genome annotation was adjusted accordingly in regard to gene location and splicing patterns. The LAT of Simplexvirus, such as HSV-1 (18) and HSV-2 (19), is expressed from the unique long inverted repeats, while the LR of the Varicellovirus, such as VZV (20), BHV-1 (11), and pseudorabies virus (PrV) (15), is expressed from unique short inverted repeats. Based on sequence alignment of conserved regions for the DNA polymerase and terminase genes, the cyprinid herpesviruses are closely related to anguillid herpesvirus 1 (AngHV-1) (Waltzek et al. At different times post-inoculation, six infected fish were randomly selected per tank, euthanized and dissected. This study represents the first report of LAMP assay for CyHV-2 detection in cultured gibel carp, which has great potential use in both laboratory and field diagnosis of the disease. It is likely that cyhv3Il10 helps to avoid immune surveillance and elimination by specifically targeting components of the carp immune system. Standard curve for quantitation of ORF81.

Despite their adult appearance, the immune system of fingerlings is not yet fully mature and functional. #AY939864), intercapsomeric triplex (ITP, Accession no. Figure 2 The portal of entry of CyHV-3 in carp analysed by bioluminescence imaging. Biocontrol viruses need to be both readily transmissible and highly virulent, requirements that are complicated by a possible evolutionary trade-off between these two variables [12]. However, the assumptions that are made to sharpen discrimination may lead to the exclusion of genuine genes, especially if they are small or expressed by splicing, are translated from alternative initiation codons, have lengthy overlapping protein-coding regions, or lack similarity to other genes. The ventral lobe of the tail fin was clipped with forceps before section. Phylogenetic analysis of the KHV genome sequence led to its classification in the new family Alloherpesviridae, encompassing herpesviruses of fish and amphibians (18).

This novel virus was placed in a recently established family, Alloherpesviridae, one of the three families of herpesviruses (order: Herpesvirales; Davison et al., 2009). Cyprinid herpesvirus 3 (CyHV-3) is a lethal DNA virus that infects the common carp (Cyprinus carpio L.) and koi carp (C. This method is practical for monitoring CyHV-3 abundance in environmental water. Herpesviral hematopoietic necrosis (HVHN), caused by Cyprinid herpesvirus 2 (CyHV-2), is a disease of goldfish Carassius auratus auratus (Linnaeus, 1758) that was first reported in juvenile goldfish in Japan in the spring and autumn of 1992 and the spring of 1993 and later in Australia, Taiwan, and the USA [1, 2]. Finally, a search for host proteins in purified CyHV-3 virions indicated the potential incorporation of up to 18 distinct cellular proteins. A novel fatal disease of fish caused by cyprinid herpesvirus 3 (CyHV-3), also known as koi herpesvirus or carp interstitial nephritis and gill necrosis virus, which infects the common carp (Cyprinus carpio carpio) and ornamental koi (Cyprinus carpio koi) was reported at the end of the 1990s, and it has spread rapidly worldwide (13). The presence of cyprinid herpesvirus 2 (CyHV-2) in dead fish was demonstrated by amplification and sequencing of portions of the DNA polymerase and helicase genes, both sequences exhibiting 100% identity with CyHV-2 from Japan.

You can also find other documents related to your research within ProQuest. The CyHV3 vaccine provided substantial protective immunity against challenge exposure. Serum osmolality of the infected fish, particularly just before death, was significantly lower, suggesting that the osmotic shock consequent on the damage to the skin was the direct cause of the acute deaths. To better understand the consequences of these diseases, a key question lies in how they persist in host populations after they emerge. The nucleus of infected cells was characterized by margination of chromatin and often contained many capsids of about 110 nm in diameter with varying morphology. In this study, the whole genome sequence of a novel Chinese CyHV3 isolate (GZ11) was determined and annotated. ABSTRACT: Cyprinid herpesvirus 1 (CyHV1) infects all scaled and color varieties of common carp (Cyprinus carpio) including koi.