[Expression of matrix metalloproteinases in experimental herpes simplex virus keratitis]. – Abstract

(1978) Considerations on the Meta-Herpetic Keratitis. Immunohistochemistry, real-time PCR, and plaque assays using HSV-1 infected tissues were performed. Results: We demonstrated that receptor usage by HSV-1 limited to nectin-1 does not significantly change the spread of HSV-1 in the corneal epithelium during primary infection. A rational approach to treatment has been facilitated by a detailed classification of the various manifestations of the involvement of the anterior segment of HSV. To study the pathogenesis of HSK in the natural host species, we measured local HSV-specific T-cell responses in HSK corneas removed at transplant surgery (n = 5) or control corneas (n= 2). These results indicate that antiviral antibodies can affect the cellular immune response in herpesvirus infections by modulating the lymphoproliferative response to herpes antigens. It may be spread to an infant during childbirth.

MgK and NgK viruses, and not the RgK virus, showed a reduced extent of explant reactivation at the lower dose of ocular infection but not at the higher dose. Infection of human ARPE-19 cells with hrR3 resulted in increased IL-6 expression and secretion 3 to 4 hours post-infection. Both herpes stromal keratitis (HSK) and HSV endotheliitis can present clinically with stromal opacity and, therefore, may be difficult to distinguish. We describe the salient clinical features which can help arrive at a diagnosis to begin appropriate treatment immediately, prior to the laboratory report. MgK and NgK viruses, and not the RgK virus, showed a reduced extent of explant reactivation at the lower dose of ocular infection but not at the higher dose. We provide a brief overview of the pathogenesis of HSK and corneal immune privilege and focus on HSK to highlight the lymphangiogenesis that occurs in response to HSV-1 corneal infection, considering both the viral and host immune contributors to this process. Finally, when administered during primary infection, (Z-LL)2 ketone inhibitor reduced HSV-1 replication in the eyes of ocularly infected mice.


In addition, the presence of anti–HSV-1 gD and gK antibodies in the sera of all patients also was determined by ELISA using gD and gK antigens. Mice were ocularly infected with HSV-1 to evaluate the effects of restricted innate or adaptive TGF-β signaling during acute and latent infections. The initial exploration gave a VA of 1 in the right eye (RE) and 0.1 in LE, normal biomicroscopy in RE with intense conjunctival injection in LE and lackluster corner with central predominance with slight edema and negative fluorescein staining, without Tyndall, synechiae, keratic precipitates or secretion. One group of rabbits received left-eye intracameral inoculation of UV-inactivated herpes simplex virus (HSV)-1 (strain McKrae). It may be spread to an infant during childbirth. Among clinically defined viral keratitis cases, a negative viral swab was predominantly (93 %) observed in cases with subepithelial inflammation and was significantly associated with an increased duration of symptoms (p = 0.003). Current research demonstrates the potential of anti-lymphangiogenic therapy to decrease and prevent herpes-induced lymphangiogenesis.

These results also suggest that SPP plays an important role in viral replication and possibly virus pathogenesis. Specular microscopy performed thereafter demonstrated relatively normal endothelial cell counts. Bottom line- nothing you’ve mentioned suggests herpes to me. See our Privacy Policy and User Agreement for details. It may be spread to an infant during childbirth. Reduced visual acuity never occurs in simple conjunctivitis. Our data are in line with observations reporting increased susceptibility of HIV-infected individuals to viral keratitis [7].

HSV-1 encodes at least 85 genes and 12 of these genes code for glycoproteins (Barnett et al., 1992; Ghiasi et al., 1998; McGeoch et al., 1988; Spear, 2004; Spear et al., 2000), one of which is glycoprotein K (gK) (Ghiasi et al., 1994b; Hutchinson et al., 1992; McGeoch et al., 1988). Among clinically defined viral keratitis cases, a negative viral swab was predominantly (93 %) observed in cases with subepithelial inflammation and was significantly associated with an increased duration of symptoms (p=0.003). MgK and NgK viruses, and not the RgK virus, showed a reduced extent of explant reactivation at the lower dose of ocular infection but not at the higher dose. Therefore, confirmation by microscopic examination and culture of the clinical samples remain the gold standard for etiological diagnosis. Finally, when administered during primary infection, (Z-LL)2 ketone inhibitor reduced HSV-1 replication in the eyes of ocularly infected mice. MgK and NgK viruses, and not the RgK virus, showed a reduced extent of explant reactivation at the lower dose of ocular infection but not at the higher dose. Based on clinical history, disease characteristics and laboratory results, 29 (63 %) patients were diagnosed as viral keratitis, including 14 (48 %) viral keratitis cases complicated by bacterial superinfection, and 17 (37 %) as bacterial keratitis.

Using two strains of mice which are susceptible (129/SVEV) and resistant (C57BL/6) to herpes simplex virus (HSV) strain KOS, (129/SVEV × C57BL/6)F2 mice were generated and examined for their disease susceptibility in terms of clinical symptoms, ocular disease, and antibody production following corneal scarification with HSV (KOS).