Ectopic expression of herpes simplex virus-thymidine kinase gene in human non-small cell lung cancer cells

Another such invagination with two nuclear pores was illustrated in . Protein expression levels of both p21 and p27 were increased after viral reactivation. Serum starvation for an extended time was avoided, as it affected cell viability. As a result of splicing, an 866-nt intron containing the entire ORF49 was removed (Fig. The ability of the vGPCR to signal in a ligand-dependent and -independent fashion may suggest modulation of the signalling profile of vGPCR in the microenvironment of KSHV tumours, which may contribute to tumourigenesis and/or viral replication. Intratumor injection of DC alone resulted in a significant (see footnotes to Fig. Activated STAT3 contributed to cell proliferation and angiogenesis in a KSHV tumor model in mice (Ma et al., 2013).

Consequently, replication-defective HSV vectors mirror the ability of the wild-type virus to undergo retrograde axonal transport and deliver the vector genome, and inserted transgene cassettes, to the nerve cell nucleus. Briefly, cells were treated with both 1 mM hydroxyurea and 50 μM BrdU for 12 h after 3 days of serum withdrawal and then doubly labeled with antibodies against MEQ (rabbit polyclonal antibodies) and BrdU (mouse MAb). As in HHV-6A-infected cells, transfected U14 localized to nuclear dots. Injection of 1 × 105 wild-type tumor cells into one flank of mice, followed 3 days later by injections of 1 × 105 irradiated DISC-HSV β-GAL or GM-CSF–transduced tumor resulted in significantly decreased tumor growth in the latter group (P < .001; Fig 10). Ov, ovary; UtH, uterine horn; Bd, body of the uterus; Cx, cervix; Vg, Vagina. Theoretically, KSHV lytic cycle replication by HSV-1 would subsequently produce infectious progeny virions. Cells were released from the G1 block by replacing Ile- or serum-free medium with medium supplemented with 10% nondialyzed FBS, 0.802 mM Ile, and 2 mM Gln.

HSV-1 DNA was detected using the JK fragment from the HSV-1 EcoRI library (42), a generous gift from the late P. Figure 1B shows the presence of LANA in these cells. The protein was designated p78 since an antiserum raised against a bacterial fusion protein containing the original 370 amino acids reacted with two major polypeptide bands with an apparentM r of 78,000 in an immunoblot (see Materials and Methods and below). At the indicated times after infection, the medium was removed, monolayers were washed with cold PBS, and cells were scraped into 1.0 ml of DNA extraction buffer (0.5% sodium dodecyl sulfate [SDS] and 50 μg of proteinase K/ml in TEN buffer [10 mM Tris · Cl, 25 mM EDTA, 100 mM NaCl, pH 8.0]). Generation of figures.Figures 1 to 4 and 6 to 7 were created using Microsoft PowerPoint. After a 1-h incubation on ice, cells were washed twice in PBS-0.1% BSA-0.01% azide and analyzed on a FACSCalibur instrument using Cellquest software (Becton-Dickinson, Oxford, United Kingdom). In contrast, the ORF73 (LANA) probe only detects the 6.0-kb LT1 transcript.

J∆NI7GFP-infected cells showed abundant, viral dose-dependent EGFP expression whereas J∆NI6GFP infection produced limited expression, even at the highest dose. Before rehybridization with a different probe, the membranes were stripped at 80°C in 10 mM Tris-HCl (pH 8) containing 1% SDS. Serial dilutions of PCR product across 8 logs were used to determine the efficiency of PCR amplification for each primer set under the above conditions so that the relative quantitation could be adjusted as defined by the Pfaffl method. All disabled viruses were propagated on BHK-derived 27/12/M:4 complementing cell lines as previously described (58). To determine the effects of overexpressed proteins on virus reactivation efficiencies, iSLK-BAC16 cells were transfected for 24 h and the lytic cycle was induced by doxycycline treatment for a further 24 h. While the frequency of spleen cells carrying the latent viral genome was comparable in B6 and B-cell−/− mice (Fig. VZV infected cells display Cdk activity even though p21 and p27 are induced.

Other primary antibodies used were mouse anti-FLAG (M2; Sigma-Aldrich), rabbit anti-FLAG (Bethyl Laboratories), and rabbit anti-GMIP (ab133975; Abcam). Determination of viral releaseVero cell monolayers were exposed to HSV-1 at a MOI of 0.1 PFU per cell for 1 h, and then the monolayers were washed three times with PBS and grown in cultures with maintenance medium at 37°C. Plasmid pECFP-Rab11S25N, encoding a transdominant-negative form of Rab11 (80) fused to enhanced cyan fluorescent protein (ECFP) was kindly provided by J. For example, in HH-B2 PEL cells, KSHV is inducible by butyrate but not by phorbol esters or azacytidine (17, 18), whereas TPA induces the KSHV lytic cycle in several other cell lines. Dephosphorylation of Thr14/Tyr15 by the protein phosphatase Cdc25 eventually activates the Cdc2–cyclin B1 complex, allowing progression to mitosis (48). Prions The bad kind of Prions are proteins that attack other proteins, converting them into something like themselves. Further, while early viral gene expression was not suppressed following JNK inhibition, viral replication remained sensitive to JNK inhibition for several hours after infection of target cells.