Detection of Caprine herpesvirus 1-specific antibodies in goat sera using an enzyme-linked immunosorbent assay and

Goat 1 was euthanized, and her ganglia were immediately collected and frozen. Restriction endonuclease analysis indicates that there are different strains but these are not geographically clustered. In this paper, we report the results of a study of the latency sites of CpHV.1 in goats by using the PCR assay. Blood samples were aseptically obtained by the jugular vein, and after clotting, were centrifuged at 1,500 × g for 10 min.c Serum samples were collected and stored at −20°C until tested. The results showed a high seroprevalence in the southern part of Corsica (Corse-du-Sud district), but CpHV-1 infection was not identified in the few mainland districts studied [26,27]. The results presented here support the hypothesis that the male goat is involved in the transmission of CHV-1. Alteration of the envelope lipid composition affected virus entry and a noticeable reduction in virus infectivity was detected in the presence of 15 mM MβCD.

The results presented here support the hypothesis that the male goat is involved in the transmission of CHV-1. Protection against infection depended on the number of injections of vaccine and the route of challenge. Thus, a vaccine composed of inactivated CpHV-1 plus MF59™ as adjuvant was strongly immunogenic and induced effective immunity against vaginal CpHV-1 infection in goats. Virus-induced apoptosis was reduced by Z-VAD-FMK, an aspecific caspase inhibitor, by AC-DEVD-CHO (caspase-3-specific) and AC-VEID-CHO (caspase-6-specific) treatment. The serosurvey was carried out in 9564 goats (275 herds) using bovine herpesvirus 1 (BoHV-1) glycoprotein B and E ELISAs. To analyse the safety and the efficacy of this marker vaccine, two groups of three goats served as controls: one immunised with a virulent CpHV-1 and one uninoculated until the challenge. A seroprevalence survey from one of the deer farms showed a high rate of subclincal infection in the deer population.

MβCD treatment did not prejudice virus binding to cells, while a dose-dependent reduction of the virus yield was observed at the virus entry stage, and 30 mM MβCD reduced infectivity evidently. RESULTS: Viral DNA was variably found in all five pairs of sacral ganglia, with a more frequent involvement of the third and fourth pair. Results demonstrate, for the first time, CpHV-1 infection in goat herds on the French mainland. Accessed on November 15, 2012). In one goat herpes-like lesions appeared on the vulvar area on PID 7. As a basis for further studies, we re-evaluated observations concerning experimental infections with BHV1 and CapHV1 in the natural and the foreign hosts. Concanavalin A-stimulated caprine peripheral blood mononuclear cells (PBMCs) cultures were studied and they exhibited high levels of soluble IL-4 and high frequencies of IFN-gamma secreting cells.

Clinical reaction was severe in the kids, but it was very mild in the lambs. On the basis of sequence data for the gB gene, a nested PCR combined with restriction enzyme analysis (REA) of the PCR products was developed for the simultaneous detection and identification of the viruses that were studied. Connect to ProQuest Once connected, you can view documents in full as well as cite, email or print them. Cidofovir antiherpetic activity was evaluated in goats infected experimentally by the vaginal route with CpHV-1 and then treated locally at different times after infection. Caprine herpesvirus 1 (CpHV-1) is an alphaherpesvirus interfering with goat reproductive performances. Here, the bovine herpesvirus 4 (BoHV-4) based vector derived from an apathogenic isolate of BoHV-4 and expressing the immunodominant CpHV-1 glycoprotein D (BoHV-4-A-gDcpgD106ΔTK) was constructed and its ability to protect goats against CpHV-1 induced genital disease evaluated. The lesions healed rapidly with only two goats showing lesions two weeks after the disease was first detected.

To evaluate the efficacy of mucosal vaccination with a beta-propiolactone inactivated caprine herpesvirus-1 (CpHV-1) vaccine, goats received vaginal administrations of two 7-day cycles at 2 weeks intervals. Fc) diluted in PBST (1:1,000) were added to each well. In a herd of 244 dairy and meat goats, physical examinations were performed in does and bucks, presenting vulvar and preputial lesions. CpHV-1 is widespread and especially in Mediterranean countries as Greece, Italy and Spain. Virus was reisolated from vaginal swabs from the 3rd to the 12th day post-treatment with DMS and from nasal swabs for 2 days (6th and 7th day post-treatment). haemolytica alone. Following two subcutaneous immunizations, goats developed high titers of CpHV-1-specific serum and vaginal IgG and high serum virus neutralization (VN) titers.

Initial experiments in Madin Darby bovine kidney (MDBK) cells revealed that CpHV-1 infection induced apoptotic features like chromatin condensation and DNA laddering. PMCID: PMC2918870 This article has been cited by other articles in PMC.Abstract Caprine herpesvirus 1 (CpHV-1) is responsible for vaginal and respiratory disease in goats. A. Two adult goats seropositive to caprine herpesvirus 1 were treated with dexamethasone for 6 days to reactivate the virus. cap·rine  (kăp′rīn′)adj. From goat and bucks with genital disorders and abortions the attempts for isolation of caprine herpesvirus 1 (CHV 1) were carried out.