Bovine Herpesvirus 1 Downregulates the Expression of Bovine MHC Class I Molecules

Vaccinated calves in the BHV-1 study were protected from clinical disease and fever, and had significantly reduced duration of nasal virus shedding. However, in select species, especially Old World apes, MLV vaccines have received sufficient use to warrant consideration based on the serious risk of morbidity and mortality. Additionally, they are simple to design and economical to produce, thus making them attractive as veterinary vaccines (5). There was no significant difference in SN titer among groups on any day. Another disadvantage of killed vaccines is the relatively short duration of immunity (7). Get a printable copy (PDF file) of the complete article (1.5M), or click on a page image below to browse page by page. Approximately 80-90% of herds have evidence of past exposure to the virus with one or more animals having antibodies to BHV-1 infection.

Additionally, they are simple to design and economical to produce, thus making them attractive as veterinary vaccines (5). Get a printable copy (PDF file) of the complete article (2.7M), or click on a page image below to browse page by page. Following challenge with BHV-1, calves immunized with the recombinant NDVs had lower titers and earlier clearance of challenge virus compared to the empty vector control, and reduced disease was observed with rLaSota/gDFL. Our current goal is to test the use of interferon-gamma (IFN-gamma) as an attenuating and adjuvant gene in live attenuated BHV-1 vaccines, capable of limiting the spread of infection and preventing the establishment of latency. The editor of the journal is Dr. CpG ODN-treated lambs also displayed a transient reduction in viral shedding on day 2 postinfection (p < 0.05), which correlated (p < 0.03) with serum 2'5'-A synthetase levels on the day of viral challenge. These results show that the BHV-1 vaccine evaluated here did not confer protection to BHV-5 in rabbits.
haemolytica from the lungs. Postchallenge nasal shedding of BHV-1 occurred only in controls and those vaccinated at 3 and 4 mo of age. Heterokaryon assays demonstrated that VP8 is also capable of shuttling between the nucleus and cytoplasm of the cell. Two doses of MLV BHV-1 (days 0 and 28) in some cases induced serum BHV-1 antibodies to higher levels and greater duration than one dose. Thiry, E. In contrast, after intradermal delivery no effect of co-administration of IL-12 encoding plasmid was observed. A 2 log(10) reduction in the peak viral titre was observed and the challenge virus excretion lasted 2 days more in immunised than in control goats.

With parts of Europe being BHV-1 free, the ability to differentiate infected from vaccinated animals has become critical as a trading tool. Of the inactivated vaccines, the gE-negative vaccine induced a better clinical protection than the gD-subunit vaccine. First, the immune response generated by i.m. The vaccinates also shed significantly less virus and for a shorter period of time (mean = 5.5 days) than the controls (mean = 9 days). injected plasmid expressing a secreted form of gD (tgD) was determined and found to be very similar in magnitude to the response induced by gD-expressing plasmid. Total leukocytes were increased by rBoIL-1 beta, primarily by causing neutrophilia and monocytosis; CD4/CD8 ratios tended to be increased in rBoIL-1 beta-treated animals. Clinical signs, immune responses, and nasal shedding of virus were monitored for 14 days after challenge.

In the first experiment, a group of calves was inoculated with 265gE− and challenged with wild type virus 21 days post-inoculation. All cattle, controls and vaccinees, excreted virus. Mice and cattle injected with plasmids encoding bovine herpesvirus 1 (BHV-1) glycoproteins developed gene-specific antibody responses capable of neutralizing BHV-1. One of the experimental groups had been previously inoculated with a live commercial vaccine but even this failed to elicit a strong immunological response. Although antibodies have been correlated with protection and recovery from BHV-1 infection, the cell-mediated immune response is also a critical defense mechanism because cell-to-cell spread occurs before hematogenous spread. Abstract A field trial was conducted to determine the effect of vaccination with an inactivated bovine herpesvirus 1 (BHV1) gE-negative marker vaccine on the milk production of dairy cows. To study possible reactivation and to quantify subsequent transmission of a live gE-negative bovine herpesvirus 1 (BHV1) vaccine strain in cattle populations, four experiments were performed.

For this study, the intercellular trafficking ability of bovine herpesvirus 1 (BHV-1) VP22 was applied to improve the efficacy of a DNA vaccine in calves. There are no comments yet. Four DNA vaccines against BoHV-1 were evaluated for their efficacy in calves. A field trial was conducted to determine the effect of vaccination with an inactivated bovine herpesvirus 1 (BHV1) gE-negative marker vaccine on the milk production of dairy cows. The study in BHV-1 naïve calves investigated the effect of intramuscularly (IM) administered BHV-1 neutralising bovine immunoglobulin on the efficacy of a live intranasally (IN) administered BHV-1 vaccine.